Defined sequence modules and an architectural element cooperate to promote initiation at an ectopic mammalian chromosomal replication origin.

ABSTRACT: A small DNA fragment containing the high-frequency initiation region (IR) ori-beta from the hamster dihydrofolate reductase locus functions as an independent replicator in ectopic locations in both hamster and human cells. Conversely, a fragment of the human lamin B2 locus containing the previously mapped IR serves as an independent replicator at ectopic chromosomal sites in hamster cells. At least four defined sequence elements are specifically required for full activity of ectopic ori-beta in hamster cells. These include an AT-rich element, a 4-bp sequence located within the mapped IR, a region of intrinsically bent DNA located between these two elements, and a RIP60 protein binding site adjacent to the bent region. The ori-beta AT-rich element is critical for initiation activity in huma

SUBMITTER: Altman AL 

PROVIDER: S-EPMC400449 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature