Rem2 in the bullfrog (Rana catesbeiana): Patterns of expression within the central nervous system and brain expression at different ontogenetic stages.

ABSTRACT: Rem2 is a member of the RGK (Rem, Rad, and Gem/Kir) subfamily of the Ras superfamily of GTP binding proteins. In mammals, Rem2 has been found to be unique in not only its structure, but also its tissue specificity, as it is the first member to be found at high levels in neuronal tissue. Because Rem2 has previously been implicated in neuronal cell proliferation, and amphibians maintain relatively high neuronal proliferative activity as adults, we sought to isolate and acquire the full-length sequence of the rem2 gene from the brain of the bullfrog (Rana catesbeiana). Furthermore, we used real time PCR (rtPCR) to characterize its tissue specificity, regional brain expression, and brain expression levels at different stages of development. Deduced amino acid sequence analysis showed that the

SUBMITTER: DeRocher MM 

PROVIDER: S-EPMC4005034 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature