A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia.

ABSTRACT: Stenotrophomonas maltophilia is a multidrug-resistant nosocomial pathogen that is difficult to identify unequivocally using current methods. Accordingly, because the presence of this microorganism in a patient may directly determine the antimicrobial treatment, conventional polymerase chain reaction (PCR) and real-time PCR assays targeting 23S rRNA were developed for the specific identification of S. maltophilia. The PCR protocol showed high specificity when tested against other species of Stenotrophomonas, non-fermentative Gram-negative bacilli and 100 clinical isolates of S. maltophilia previously identified using the Vitek system.

SUBMITTER: Gallo SW 

PROVIDER: S-EPMC4005581 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature