The FurA regulon in Anabaena sp. PCC 7120: in silico prediction and experimental validation of novel target genes.

ABSTRACT: In the filamentous cyanobacterium Anabaena sp. PCC 7120, the ferric uptake regulator FurA functions as a global transcriptional regulator. Despite several analyses have focused on elucidating the FurA-regulatory network, the number of target genes described for this essential transcription factor is limited to a handful of examples. In this article, we combine an in silico genome-wide predictive approach with experimental determinations to better define the FurA regulon. Predicted FurA-binding sites were identified upstream of 215 genes belonging to diverse functional categories including iron homeostasis, photosynthesis and respiration, heterocyst differentiation, oxidative stress defence and light-dependent signal transduction mechanisms, among others. The probabilistic model proved to b

SUBMITTER: Gonzalez A 

PROVIDER: S-EPMC4005646 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature