Transcript level and sequence determinants of protein abundance and noise in Escherichia coli.

ABSTRACT: The range over which a protein is expressed, and its cell-to-cell variability, is often thought to be linked to the demand for its activity. Steady-state protein level is determined by multiple mechanisms controlling transcription and translation, many of which are limited by DNA- and RNA-encoded signals that affect initiation, elongation and termination of polymerases and ribosomes. We performed a comprehensive analysis of >100 sequence features to derive a predictive model composed of a minimal non-redundant set of factors explaining 66% of the total variation of protein abundance observed in >800 genes in Escherichia coli. The model suggests that protein abundance is primarily determined by the transcript level (53%) and by effectors of translation elongation (12%), whereas only a small

SUBMITTER: Guimaraes JC 

PROVIDER: S-EPMC4005695 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature