Identification of in vivo regulators of the Vibrio cholerae xds gene using a high-throughput genetic selection.

ABSTRACT: Vibrio cholerae, the causative agent of cholera, remains a threat to public health in areas with inadequate sanitation. As a waterborne pathogen, V. cholerae moves between two dissimilar environments, aquatic reservoirs and the intestinal tract of humans. Accordingly, this pathogen undergoes adaptive shifts in gene expression throughout the different stages of its lifecycle. One particular gene, xds, encodes a secreted exonuclease that was previously identified as being induced during infection. Here we sought to identify regulators responsible for the in vivo-specific induction of xds. A transcriptional fusion of xds to two consecutive antibiotic resistance genes was used to select transposon mutants that had inserted within or adjacent to regulatory genes and thereby caused increased exp

SUBMITTER: McDonough E 

PROVIDER: S-EPMC4005888 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature