Evidence for the involvement of type I interferon in pulmonary arterial hypertension.

ABSTRACT:

Rationale

Evidence is increasing of a link between interferon (IFN) and pulmonary arterial hypertension (PAH). Conditions with chronically elevated endogenous IFNs such as systemic sclerosis are strongly associated with PAH. Furthermore, therapeutic use of type I IFN is associated with PAH. This was recognized at the 2013 World Symposium on Pulmonary Hypertension where the urgent need for research into this was highlighted.

Objective

To explore the role of type I IFN in PAH.

Methods and results

Cells were cultured using standard approaches. Cytokines were measured by ELISA. Gene and protein expression were measured using reverse transcriptase polymerase chain reaction, Western blotting, and immunohistochemistry. The role of type I IFN in PAH in vivo was determined usi

SUBMITTER: George PM 

PROVIDER: S-EPMC4006084 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature