Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources.

ABSTRACT: Quantitative views of cellular functions require precise measures of rates of biomolecule production, especially proteins-the direct effectors of biological processes. Here, we present a genome-wide approach, based on ribosome profiling, for measuring absolute protein synthesis rates. The resultant E. coli data set transforms our understanding of the extent to which protein synthesis is precisely controlled to optimize function and efficiency. Members of multiprotein complexes are made in precise proportion to their stoichiometry, whereas components of functional modules are produced differentially according to their hierarchical role. Estimates of absolute protein abundance also reveal principles for optimizing design. These include how the level of different types of transcription factor

SUBMITTER: Li GW 

PROVIDER: S-EPMC4006352 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature