Gene profiling of human induced pluripotent stem cell-derived astrocyte progenitors following spinal cord engraftment.

ABSTRACT: The generation of human induced pluripotent stem cells (hiPSCs) represents an exciting advancement with promise for stem cell transplantation therapies as well as for neurological disease modeling. Based on the emerging roles for astrocytes in neurological disorders, we investigated whether hiPSC-derived astrocyte progenitors could be engrafted to the rodent spinal cord and how the characteristics of these cells changed between in vitro culture and after transplantation to the in vivo spinal cord environment. Our results show that human embryonic stem cell- and hiPSC-derived astrocyte progenitors survive long-term after spinal cord engraftment and differentiate to astrocytes in vivo with few cells from other lineages present. Gene profiling of the transplanted cells demonstrates the astroc

SUBMITTER: Haidet-Phillips AM 

PROVIDER: S-EPMC4006486 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature