BMP6 reverses TGF-beta1-induced changes in HK-2 cells: implications for the treatment of renal fibrosis.

ABSTRACT:

Aim

The aim of the study was to investigate the potential role of BMP6 in TGF-beta1-mediated changes in HK-2 cells.

Methods

BMP6 was purified via heparin affinity and reverse phase liquid chromatography. The purity, specificity, and bioactivity of BMP6 were determined by SDS-PAGE, Western blot assays, and the induction of alkaline phosphatase (ALP) activity, respectively. Cell proliferation, morphology, and expression levels of alpha-SMA and E-cadherin were assessed by cell viability, microscopy, and Western blot assays, respectively. In addition, cell adhesion abilities were determined by counting the number of attached cells. The expression of fibronectin, collagen IV, matrix metalloproteinases 2 (MMP-2), and tissue inhibitors of matrix metalloproteinases 2 (TIMP-2) were a

SUBMITTER: Yan JD 

PROVIDER: S-EPMC4006648 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature