Discovery and confirmation of ligand binding specificities of the Schistosoma japonicum polarity protein Scribble.

ABSTRACT:

Background

Schistosomiasis is a chronic debilitating parasitic disease that afflicts more than 200 million individuals worldwide. Long-term administration of chemotherapy with the single available drug, praziquantel, has led to growing concerns about drug resistance. The PSD-95/Dlg/ZO-1 (PDZ) domain is an important module found in many scaffolding proteins, which has been recognized as promising targets for the development of novel drugs. However, the parasite-derived PDZ domains and their associated functions are still largely unknown.

Methodology/principal findings

The gene encoding the Schistosoma japonicum Scribble protein (SjScrib) was identified by homologous search with the S. mansoni Scrib sequence. By screening an arbitrary peptide library in yeast two-hybrid (Y2H)

SUBMITTER: Cai P 

PROVIDER: S-EPMC4006718 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature