MALDI-TOF identification of the human Gut microbiome in people with and without diarrhea in Senegal.

ABSTRACT:

Background

In Africa, there are several problems with the specific identification of bacteria. Recently, MALDI-TOF mass spectrometry has become a powerful tool for the routine microbial identification in many clinical laboratories.

Methodology/principal findings

This study was conducted using feces from 347 individuals (162 with diarrhea and 185 without diarrhea) sampled in health centers in Dakar, Senegal. Feces were transported from Dakar to Marseille, France, where they were cultured using different culture conditions. The isolated colonies were identified using MALDI-TOF. If a colony was unidentified, 16S rRNA sequencing was performed. Overall, 2,753 isolates were tested, allowing for the identification of 189 bacteria from 5 phyla, including 2 previously unknown species

SUBMITTER: Samb-Ba B 

PROVIDER: S-EPMC4006720 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature