Allele-specific genome-wide profiling in human primary erythroblasts reveal replication program organization.

ABSTRACT: We have developed a new approach to characterize allele-specific timing of DNA replication genome-wide in human primary basophilic erythroblasts. We show that the two chromosome homologs replicate at the same time in about 88% of the genome and that large structural variants are preferentially associated with asynchronous replication. We identified about 600 megabase-sized asynchronously replicated domains in two tested individuals. The longest asynchronously replicated domains are enriched in imprinted genes suggesting that structural variants and parental imprinting are two causes of replication asynchrony in the human genome. Biased chromosome X inactivation in one of the two individuals tested was another source of detectable replication asynchrony. Analysis of high-resolution TimEX pr

SUBMITTER: Mukhopadhyay R 

PROVIDER: S-EPMC4006724 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature