Profiling molecular changes induced by hydrogen treatment of lung allografts prior to procurement.

ABSTRACT:

Background

We previously demonstrated that donor treatment with inhaled hydrogen protects lung grafts from cold ischemia/reperfusion (I/R) injury during lung transplantation. To elucidate the mechanisms underlying hydrogen's protective effects, we conducted a gene array analysis to identify changes in gene expression associated with hydrogen treatment.

Methods

Donor rats were exposed to mechanical ventilation with 98% oxygen and 2% nitrogen or 2% hydrogen for 3 h before harvest; lung grafts were stored for 4h in cold Perfadex. Affymetrix gene array analysis of mRNA transcripts was performed on the lung tissue prior to implantation.

Results

Pretreatment of donor lungs with hydrogen altered the expression of 229 genes represented on the array (182 upregulated; 47 downre

SUBMITTER: Tanaka Y 

PROVIDER: S-EPMC4007057 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature