Aqueous two-phase system patterning of detection antibody solutions for cross-reaction-free multiplex ELISA.

ABSTRACT: Accurate disease diagnosis, patient stratification and biomarker validation require the analysis of multiple biomarkers. This paper describes cross-reactivity-free multiplexing of enzyme-linked immunosorbent assays (ELISAs) using aqueous two-phase systems (ATPSs) to confine detection antibodies at specific locations in fully aqueous environments. Antibody cross-reactions are eliminated because the detection antibody solutions are co-localized only to corresponding surface-immobilized capture antibody spots. This multiplexing technique is validated using plasma samples from allogeneic bone marrow recipients. Patients with acute graft versus host disease (GVHD), a common and serious condition associated with allogeneic bone marrow transplantation, display higher mean concentrations for four

SUBMITTER: Frampton JP 

PROVIDER: S-EPMC4007081 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature