Sodium butyrate and dexamethasone promote exocrine pancreatic gene expression in mouse embryonic stem cells.

ABSTRACT:

Aim

The feasibility of inducing endocrine pancreatic differentiation of embryonic stem (ES) cells has been well documented. However, whether ES cells possess the potential for exocrine pancreatic differentiation requires further exploration. Here, we investigated whether sodium butyrate and glucocorticoids were conducive to the exocrine pancreatic differentiation of ES cells.

Methods

E14 mouse ES cells were cultured in suspension to form embryoid bodies (EBs). These EBs were cultured in differentiating medium containing varying concentrations of sodium butyrate. The effects of activinA and dexamethasone (Dex) on exocrine differentiation were also explored. Finally, the combination of sodium butyrate, activinA, and Dex was used to promote the differentiation of exocrine pancr

SUBMITTER: Ren M 

PROVIDER: S-EPMC4007190 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature