Salivary proteomics in bisphosphonate-related osteonecrosis of the jaw.

ABSTRACT:

Objective

The objective of this study was to identify differentially expressed salivary proteins in bisphosphonate-related osteonecrosis of the jaw (BRONJ) patients that could serve as biomarkers for BRONJ diagnosis.

Subjects and methods

Whole saliva obtained from 20 BRONJ patients and 20 controls were pooled within groups. The samples were analyzed using iTRAQ-labeled two-dimensional liquid chromatography-tandem mass spectrometry.

Results

Overall, 1340 proteins were identified. Of these, biomarker candidates were selected based on P-value (<0.001), changes in protein expression (≥1.5-fold increase or decrease), and unique peptides identified (≥2). Three comparisons made between BRONJ and control patients identified 200 proteins to be differentially expressed in BRONJ

SUBMITTER: Thumbigere-Math V 

PROVIDER: S-EPMC4007366 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature