Linkage mapping and expression analysis of miRNAs and their target genes during fiber development in cotton.

ABSTRACT:

Background

MicroRNAs (miRNAs) are small, endogenously expressed, non-coding RNA molecules involved in gene transcription and expression that combine with specific mRNA site of target genes to inhibit protein synthesis or degrade mRNA. Since the first plant miRNA was reported in 2002, numerous new miRNAs and their targets have been discovered via high-throughput sequencing and computational approaches. However, the genetic variation of miRNA genes is poorly understood due to the lack of miRNA-specific DNA markers.

Results

To study the genetic variation and map miRNAs and their putative target genes in cotton, we designed specific primers based on pre-miRNAs and published putative target genes. A total of 83 pre-miRNA primers and 1,255 putative target gene primers were surveye

SUBMITTER: Chen X 

PROVIDER: S-EPMC4007520 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature