A transcriptomic reporter assay employing neutrophils to measure immunogenic activity of septic patients' plasma.

ABSTRACT:

Background

There are diverse molecules present in blood plasma that regulate immune functions and also present a potential source of disease biomarkers and therapeutic targets. Genome-wide profiling has become a powerful method for assessing immune responses on a systems scale, but technologies that can measure the plasma proteome still face considerable challenges. An alternative approach to direct proteome assessment is to measure transcriptome responses in reporter cells exposed in vitro to plasma. In this report we describe such a "transcriptomic reporter assay" to assess plasma from patients with sepsis, which is a common and severe systemic infectious process for which physicians lack efficient diagnostic or prognostic markers.

Methods

Plasma samples collected from pat

SUBMITTER: Khaenam P 

PROVIDER: S-EPMC4007645 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature