A novel polyamide SL-A92 as a potential fungal resistance blocker: synthesis and bioactivities in Candida albicans.

ABSTRACT:

Aim

To synthesize a novel polyamide SL-A92 and evaluate its bioactivity against drug resistance in Candida albicans.

Methods

SL-A92 was synthesized using N-hydroxybenzotriazole (HOBT)/N,N'-dicyclohexylcarbodiimide (DCC) in solution phase. Its antifungal activities and effects on strain growth were tested using the micro-broth dilution method and growth curves, respectively. Induced drug resistance in the C. albicans collection strain SC5314 was obtained by incubation with fluconazole (12 microg/mL) for 21 passages. Meanwhile, incubations with SL-A92 plus fluconazole were also carried out in SC5314 strains, and the MIC(80)s were used to evaluate the inhibitory effects of SL-A92 on drug resistance during the induction process. Real time RT-PCR was performed to investigate the

SUBMITTER: Zhu SL 

PROVIDER: S-EPMC4007733 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature