IL-12 and IL-27 regulate the phagolysosomal pathway in mycobacteria-infected human macrophages.

ABSTRACT:

Background

The cytokine environment at the site of infection is important to the control of mycobacteria by host macrophages. During chronic infection immunosuppressive cytokines are likely to favor mycobacterial growth, persistence, and an avoidance of proper antigen processing and presentation. The activity of interleukin (IL)-27 toward macrophages is anti-inflammatory and this compromises control of mycobacteria. Modulation of the cytokine environment may enhance both protective and vaccine-induced responses.

Results

In this study we showed that supplying IL-12 and neutralizing IL-27 enhanced acidification and fusion of mycobacterial-containing phagosomes with lysosomes. This was achieved by phagosomal acquisition of vacuolar ATPase (V-ATPase) and CD63. Both V-ATPase and

SUBMITTER: Jung JY 

PROVIDER: S-EPMC4007735 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature