Gold (I) N-heterocyclic carbene complex inhibits mouse melanoma growth by p53 upregulation.

ABSTRACT:

Background

Cancer treatment using gold (I) complexes is becoming popular. In this study, a gold (I) N-heterocyclic complex designated as complex 3 was synthesized, its cytotoxicity was examined, and its anti-melanoma activity was evaluated in vitro and in vivo.

Methods

Viability of cancer cells was determined by MTT assay upon treatment with various concentrations of a gold (I) N-heterocyclic carbene complex (complex 3) in a dose and time dependent manner. Mouse melanoma cells B16F10 were selected for further apoptotic studies, including flowcytometric analysis of annexin binding, cell cycle arrest, intracellular ROS generation and loss in the mitochondrial membrane potential. ELISA based assays were done for caspase activities and western blots for determining the expressio

SUBMITTER: Nandy A 

PROVIDER: S-EPMC4007776 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature