Ligand-regulated heterodimerization of peroxisome proliferator-activated receptor α with liver X receptor α.

ABSTRACT: Peroxisome proliferator-activated receptor α (PPARα) and liver X receptor α (LXRα) are members of the nuclear receptor superfamily that function to regulate lipid metabolism. Complex interactions between the LXRα and PPARα pathways exist, including competition for the same heterodimeric partner, retinoid X receptor α (RXRα). Although data have suggested that PPARα and LXRα may interact directly, the role of endogenous ligands in such interactions has not been investigated. Using in vitro protein-protein binding assays, circular dichroism, and co-immunoprecipitation of endogenous proteins, we established that full-length human PPARα and LXRα interact with high affinity, resulting in altered protein conformations. We demonstrated for the first time that the affinity of this interaction and t

SUBMITTER: Balanarasimha M 

PROVIDER: S-EPMC4007980 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature