Establishment of new clonal pancreatic β-cell lines (MIN6-K) useful for study of incretin/cyclic adenosine monophosphate signaling.

ABSTRACT: Incretin/cyclic adenosine monophosphate (cAMP) signaling is critical for potentiation of insulin secretion. Although several cell lines of pancreatic β-cells are currently available, there are no cell lines suitable for investigation of incretin/cAMP signaling. In the present study, we have newly established pancreatic β-cell lines (named MIN6-K) from the IT6 mouse, which develops insulinoma. MIN6-K8 cells respond to both glucose and incretins, such as glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), as is the case in pancreatic islets, whereas MIN6-K20 cells respond to glucose, but not to incretins. Despite the difference in incretin-potentiated insulin secretion between these two cell lines, the accumulation of cAMP after stimulation of GLP-1 is com

SUBMITTER: Iwasaki M 

PROVIDER: S-EPMC4008005 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature