Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers.

ABSTRACT: X-ray free-electron laser (XFEL) sources enable the use of crystallography to solve three-dimensional macromolecular structures under native conditions and without radiation damage. Results to date, however, have been limited by the challenge of deriving accurate Bragg intensities from a heterogeneous population of microcrystals, while at the same time modeling the X-ray spectrum and detector geometry. Here we present a computational approach designed to extract meaningful high-resolution signals from fewer diffraction measurements.

SUBMITTER: Hattne J 

PROVIDER: S-EPMC4008696 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature