Ontology highlight
ABSTRACT:
SUBMITTER: Elling U
PROVIDER: S-EPMC4008724 | biostudies-literature | 2011 Dec
REPOSITORIES: biostudies-literature
Cell stem cell 20111201 6
All somatic mammalian cells carry two copies of chromosomes (diploidy), whereas organisms with a single copy of their genome, such as yeast, provide a basis for recessive genetics. Here we report the generation of haploid mouse ESC lines from parthenogenetic embryos. These cells carry 20 chromosomes, express stem cell markers, and develop into all germ layers in vitro and in vivo. We also developed a reversible mutagenesis protocol that allows saturated genetic recessive screens and results in h ...[more]