Variation of the detergent-binding capacity and phospholipid content of membrane proteins when purified in different detergents.

ABSTRACT: Purified membrane proteins are ternary complexes consisting of protein, lipid, and detergent. Information about the amounts of detergent and endogenous phospholipid molecules bound to purified membrane proteins is largely lacking. In this systematic study, three model membrane proteins of different oligomeric states were purified in nine different detergents at commonly used concentrations and characterized biochemically and biophysically. Detergent-binding capacities and phospholipid contents of the model proteins were determined and compared. The insights on ternary complexes obtained from the experimental results, when put into a general context, are summarized as follows. 1), The amount of detergent and 2) the amount of endogenous phospholipids bound to purified membrane proteins are d

SUBMITTER: Ilgu H 

PROVIDER: S-EPMC4008799 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature