Zebrafish cardiac muscle thick filaments: isolation technique and three-dimensional structure.

ABSTRACT: To understand how mutations in thick filament proteins such as cardiac myosin binding protein-C or titin, cause familial hypertrophic cardiomyopathies, it is important to determine the structure of the cardiac thick filament. Techniques for the genetic manipulation of the zebrafish are well established and it has become a major model for the study of the cardiovascular system. Our goal is to develop zebrafish as an alternative system to the mammalian heart model for the study of the structure of the cardiac thick filaments and the proteins that form it. We have successfully isolated thick filaments from zebrafish cardiac muscle, using a procedure similar to those for mammalian heart, and analyzed their structure by negative-staining and electron microscopy. The isolated filaments appear we

SUBMITTER: Gonzalez-Sola M 

PROVIDER: S-EPMC4008832 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature