The study of the frequency effect of dynamic compressive loading on primary articular chondrocyte functions using a microcell culture system.

ABSTRACT: Compressive stimulation can modulate articular chondrocyte functions. Nevertheless, the relevant studies are not comprehensive. This is primarily due to the lack of cell culture apparatuses capable of conducting the experiments in a high throughput, precise, and cost-effective manner. To address the issue, we demonstrated the use of a perfusion microcell culture system to investigate the stimulating frequency (0.5, 1.0, and 2.0 Hz) effect of compressive loading (20% and 40% strain) on the functions of articular chondrocytes. The system mainly integrates the functions of continuous culture medium perfusion and the generation of pneumatically-driven compressive stimulation in a high-throughput micro cell culture system. Results showed that the compressive stimulations explored did not have a

SUBMITTER: Lin WY 

PROVIDER: S-EPMC4009256 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature