Leptin level and oxidative stress contribute to obesity-induced low testosterone in murine testicular tissue.

ABSTRACT:

Objective

This study evaluated the effects of obesity on the function of reproductive organs in male mice and the possible mechanism of male secondary hypogonadism (SH) in obesity.

Methods

Ninety-six mice were randomly assigned to three groups: the control group, diet-induced obesity group, and diet-induced obesity resistant group for 8 weeks and 19 weeks. The effects of short- and long-term high-fat diet on the reproductive organs were determined by measuring sperm count and motility, relative testis weight, testosterone level, pathological changes and apoptosis of Leydig cells. Oxidative stress was evaluated by determining malondialdehyde, H2O2, NO levels, and GSH in testis tissues. CAT, SOD, GSH-Px and Nrf2 mRNA were measured by real-time PCR.

Results

Short- and lo

SUBMITTER: Zhao J 

PROVIDER: S-EPMC4009340 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature