Spectrum of LKB1, EGFR, and KRAS mutations in chinese lung adenocarcinomas.

ABSTRACT:

Introduction

Somatic LKB1 mutations are found in lung adenocarcinomas at different frequencies in Caucasian and East Asian (Japanese and Korean) populations. This study was designed to characterize the frequency of LKB1 mutations, their relationship to EGFR and KRAS mutations, and their associated clinicopathologic characteristics in Chinese patients.

Methods

Two hundred thirty-nine lung adenocarcinomas consecutively collected from October 2007 to July 2009 were dissected into 3 to 4 small (3 mm) pieces for histopathological analyses of tumor content. Genomic DNA and/or cDNA from 86 samples with more than 70% tumor content were used for sequencing of LKB1 (exons 1-9), EGFR (exons 18-21), and KRAS (exon 2). LKB1 germline mutation status was determined by sequencing of genomic

SUBMITTER: Gao B 

PROVIDER: S-EPMC4009449 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature