Derivation of ES-like cell from neonatal mouse testis cells in autologous sertoli cells co-culture system.

ABSTRACT:

Background

Spermatogonial stem cell (SSC) is a self-renewing population of male adult stem cell. SSCs have a differentiation potential which are similar to embryonic stem cells. These Embryonic stem like (ES-like) cells can be a potential source for pluripotent cells for stem cell-based therapy.

Objective

This study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testis Materials and Methods: Isolated testicular cells were cultured in DMEM/F12. Characteristics of the isolated cells and obtained ES-like cell were immune-cytochemically confirmed by examining the presence of PLZF, vimentin, Oct4 and Nanog protein. Expression of the pluripotency and germ-cell specific genes was analyzed by qPCR in derived ES-like colo

SUBMITTER: Asadi MH 

PROVIDER: S-EPMC4009581 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature