A tissue graft model of DNA damage response in the normal and malignant human prostate.

ABSTRACT:

Purpose

DNA damage responses are relevant to prostate cancer initiation, progression and treatment. Few models of the normal and malignant human prostate that maintain stromal-epithelial interactions in vivo exist in which to study DNA damage responses. We evaluated the feasibility of maintaining tissue slice grafts at subcutaneous vs subrenal capsular sites in RAG2(-/-)γC(-/-) mice to study the DNA damage responses of normal and malignant glands.

Materials and methods

We compared the take rate and histology of tissue slice grafts from fresh, precision cut surgical specimens that were maintained for 1 to 4 weeks in subcutaneous vs subrenal capsular sites. Induction of γH2AX, p53, ATM and apoptosis was evaluated as a measure of the DNA damage response after irradiation.

Re

SUBMITTER: Af Hallstrom TM 

PROVIDER: S-EPMC4009951 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature