Genome-wide DNA methylation patterns in naive CD4+ T cells from patients with primary Sjogren's syndrome.

ABSTRACT:

Objective

Primary Sjögren's syndrome (SS) is a systemic autoimmune disease with incompletely understood etiology. This study was undertaken to investigate the role of epigenetic dysregulation in the pathogenesis of primary SS.

Methods

A genome-wide DNA methylation study was performed in naive CD4+ T cells from 11 patients with primary SS compared to age-, sex-, and ethnicity-matched healthy controls. Cytosine methylation was quantified using the Illumina Infinium HumanMethylation450 BeadChip array, and the data were validated using bisulfite sequencing.

Results

Genome-wide analyses identified 553 hypomethylated CpG sites and 200 hypermethylated CpG sites in naive CD4+ T cells from patients with primary SS as compared to healthy controls, representing 311 hypomethylate

SUBMITTER: Altorok N 

PROVIDER: S-EPMC4009982 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature