Fluorescent live-cell imaging of metabolically incorporated unnatural cyclopropene-mannosamine derivatives.

ABSTRACT: Sugar coated: We recently developed methylcyclopropenes as low-molecular-weight tetrazine coupling partners. Here, we demonstrate that methylcyclopropenes can meet the stringent steric demands required for metabolic imaging of unnatural mannosamines on live cells. Using sequential azide-alkyne chemistry, we also demonstrate multicolor imaging of two different metabolically incorporated unnatural sugars.

SUBMITTER: Cole CM 

PROVIDER: S-EPMC4010072 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature