Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone.

ABSTRACT:

Aim

To investigate the effect of genistein on bone homeostasis in mandibular subchondral bone of rats.

Methods

Female SD rats were administered with genistein (10 and 50 mg/kg) or placebo by oral gavage for 6 weeks. Then the animals were sacrificed, and histomorphology and micro-structure of mandibular condyle were examined using HE staining and micro-CT analysis, respectively. The expression levels of alkaline phosphatase (ALP), osteocalcin (OC), osteoprotegerin (OPG), the receptor activator of nuclear factor κB ligand (RANKL) and estrogen receptors (ERs) in mandibular condyle were detected using real-time PCR. Cultured osteoblasts were prepared from rat mandibular condyle for in in vitro study. The cells were treated with genistein (10(-7) or 10(-4) mol/L) for 48 h. The ex

SUBMITTER: Li YQ 

PROVIDER: S-EPMC4010271 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature