Characterization of the immune responses elicited by baculovirus-based vector vaccines against influenza virus hemagglutinin.

ABSTRACT:

Aim

To compare the specific immune responses elicited by different baculovirus vectors in immunized mice.

Methods

We constructed and characterized two recombinant baculoviruses carrying the expression cassette for the H5N1 influenza virus hemagglutinin (HA) gene driven by either an insect cell promoter (vAc-HA) or a dual-promoter active both in insect and mammalian cells (vAc-HA-DUAL). Virus without the HA gene (vAc-EGFP) was used as a control. These viruses were used to immunize mice subcutaneously and intraperitoneally. The production of total and specific antibodies was determined by ELISA and competitive ELISA. Cytokine production by the spleen cells of immunized mice was studied using the ELISPOT assay.

Results

Both the vAc-HA and vAc-HA-DUAL vectors expressed HA

SUBMITTER: Hu ZP 

PROVIDER: S-EPMC4010374 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature