A new genetically encoded single-chain biosensor for Cdc42 based on FRET, useful for live-cell imaging.

ABSTRACT: Cdc42 is critical in a myriad of cellular morphogenic processes, requiring precisely regulated activation dynamics to affect specific cellular events. To facilitate direct observations of Cdc42 activation in live cells, we developed and validated a new biosensor of Cdc42 activation. The biosensor is genetically encoded, of single-chain design and capable of correctly localizing to membrane compartments as well as interacting with its upstream regulators including the guanine nucleotide dissociation inhibitor. We characterized this new biosensor in motile mouse embryonic fibroblasts and observed robust activation dynamics at leading edge protrusions, similar to those previously observed for endogenous Cdc42 using the organic dye-based biosensor system. We then extended our validations and o

SUBMITTER: Hanna S 

PROVIDER: S-EPMC4010534 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature