Bead arrays for antibody and complement profiling reveal joint contribution of antibody isotypes to C3 deposition.

ABSTRACT: The development of antigen arrays has provided researchers with great tools to identify reactivities against self or foreign antigens from body fluids. Yet, these approaches mostly do not address antibody isotypes and their effector functions even though these are key points for a more detailed understanding of disease processes. Here, we present a bead array-based assay for a multiplexed determination of antigen-specific antibody levels in parallel with their properties for complement activation. We measured the deposition of C3 fragments from serum samples to reflect the degree of complement activation via all three complement activation pathways. We utilized the assay on a bead array containing native and citrullinated peptide antigens to investigate the levels of IgG, IgM and IgA autoa

SUBMITTER: Ayoglu B 

PROVIDER: S-EPMC4010547 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature