Excess genistein suppresses the synthesis of extracellular matrix in female rat mandibular condylar cartilage.

ABSTRACT:

Aim

To investigate the effect of excess genistein on the extracellular matrix in mandibular condylar cartilage of female rats in vivo.

Methods

Female SD rats were administered through oral gavage with genistein (50 mg/kg) or placebo daily for 6 weeks. The morphological changes of temporomandibular joints were studied with HE staining. The expression of cartilage matrix compounds (aggrecan and collagen type II), estrogen-related molecules (aromatase, estradiol, ERα and ERβ) and proliferating cell nuclear antigen (PCNA) in mandibular condylar cartilage was detected using immunohistochemistry, ELISA and real-time PCR.

Results

The genistein treatment significantly reduced the thickness of the posterior and middle regions of mandibular condylar cartilage, and decreased the

SUBMITTER: Yu SB 

PROVIDER: S-EPMC4011151 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature