Vam3, a derivative of resveratrol, attenuates cigarette smoke-induced autophagy.

ABSTRACT:

Aim

To appraise the efficacy of Vam3 (Amurensis H), a dimeric derivative of resveratrol, at inhibiting cigarette smoke-induced autophagy.

Methods

Human bronchial epithelial cells were treated with cigarette smoke condensates, and a chronic obstructive pulmonary disease (COPD) model was established by exposing male BALB/c mice to cigarette smoke. The protein levels of the autophagic marker microtubule-associated protein 1A/1B-light chain 3 (LC3), Sirtuin 1 (Sirt1), and foxhead box O 3a (FoxO3a) were examined using Western blotting and Immunohistochemistry. LC3 punctae were detected by immunofluorescence. The levels of FoxO3a acetylation were examined by immunoprecipitation. The level of intracellular oxidation was assessed by detecting ROS and GSH-Px.

Results

Vam3 atte

SUBMITTER: Shi J 

PROVIDER: S-EPMC4011152 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature