The biphasic redox sensing of SENP3 accounts for the HIF-1 transcriptional activity shift by oxidative stress.

ABSTRACT:

Aim

To investigate the mechanisms underlying the biphasic redox regulation of hypoxia-inducible factor-1 (HIF-1) transcriptional activity under different levels of oxidative stress caused by reactive oxidative species (ROS).

Methods

HeLa cells were exposed to different concentrations of H(2)O(2) as a simple model for mild and severe oxidative stress. Luciferase reporter assay and/or quantitative real-time PCR were used to investigate the transcriptional activity. Immunoblot was used to detect protein expression. Chromatin immunoprecipitation assay was used to detect HIF-1/DNA binding. The interaction of p300 with HIF-1α or with SENP3, and the SUMO2/3 conjugation states of p300 were examined by coimmunoprecipitation.

Results

HIF-1 transcriptional activity in HeLa cells

SUBMITTER: Wang Y 

PROVIDER: S-EPMC4011156 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature