Epithelial to mesenchymal transition in murine tracheal allotransplantation: an immunohistochemical observation.

ABSTRACT:

Background

Aberrant epithelial repair is a crucial event in the airway remodeling that characterizes obliterative bronchiolitis (OB) in transplanted lungs. Recent data from experiments using epithelial cell lines and human airway tissues from lung transplant recipients suggest that epithelial to mesenchymal transition (EMT) plays an important role in OB. The aim of this study was to clarify whether EMT is involved in airway remodeling in an animal model.

Methods

We performed orthotopic tracheal transplantation from BALB/c to C57BL/6 mice with from BALC/c to BALB/c mouse grafts as controls. Five allogeneic and 3 syngeneic recipients were humanely killed at predetermined postoperative days 2-12 as well as 14 and 21. Histology was evaluated using hematoxylin-eosin (H&E) stainin

SUBMITTER: Konoeda C 

PROVIDER: S-EPMC4011642 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature