Engineering toward a bacterial "endoplasmic reticulum" for the rapid expression of immunoglobulin proteins.

ABSTRACT: Antibodies are well-established as therapeutics, and the preclinical and clinical pipeline of these important biologics is growing rapidly. Consequently, there is considerable interest in technologies to engineer and manufacture them. Mammalian cell culture is commonly used for production because eukaryotic expression systems have evolved complex and efficient chaperone systems for the folding of antibodies. However, given the ease and manipulability of bacteria, antibody discovery efforts often employ bacterial expression systems despite their limitations in generating high titers of functional antibody. Open-Cell Free Synthesis (OCFS) is a coupled transcription-translation system that has the advantages of prokaryotic systems while achieving high titers of antibody expression. Due to the

SUBMITTER: Groff D 

PROVIDER: S-EPMC4011911 | biostudies-literature | 2014 May-Jun

REPOSITORIES: biostudies-literature