Influence of artifact removal on rare species recovery in natural complex communities using high-throughput sequencing.

ABSTRACT: Large-scale high-throughput sequencing techniques are rapidly becoming popular methods to profile complex communities and have generated deep insights into community biodiversity. However, several technical problems, especially sequencing artifacts such as nucleotide calling errors, could artificially inflate biodiversity estimates. Sequence filtering for artifact removal is a conventional method for deleting error-prone sequences from high-throughput sequencing data. As rare species represented by low-abundance sequences in datasets may be sensitive to artifact removal process, the influence of artifact removal on rare species recovery has not been well evaluated in natural complex communities. Here we employed both internal (reliable operational taxonomic units selected from communities

SUBMITTER: Zhan A 

PROVIDER: S-EPMC4011962 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature