A double-taper optical fiber-based radiation wave other than evanescent wave in all-fiber immunofluorescence biosensor for quantitative detection of Escherichia coli O157:H7.

ABSTRACT: Cylindrical or taper-and-cylinder combination optical fiber probe based on evanescent wave has been widely used for immunofluorescence biosensor to detect various analytes. In this study, in contrast to the contradiction between penetration depth and analyte diameter of optical fiber probe-based evanescent wave, we demonstrate that double-taper optical fiber used in a radiation wave-based all-fiber immunofluorescence biosensor (RWAIB) can detect micron-scale analytes using Escherichia coli O157:H7 as representative target. Finite-difference time-domain method was used to compare the properties of evanescent wave and radiation wave (RW). Ray-tracing model was formulated to optimize the taper geometry of the probe. Based on a commercial multi-mode fiber, a double-taper probe was fabricated a

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC4013131 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature