Resolution of mitochondrial oxidative stress rescues coronary collateral growth in Zucker obese fatty rats.

ABSTRACT:

Objective

We have previously found abrogated ischemia-induced coronary collateral growth in Zucker obese fatty (ZOF) rats compared with Zucker lean (ZLN) rats. Because ZOF rats have structural abnormalities in their mitochondria suggesting dysfunction and also show increased production of O(2), we hypothesized that mitochondrial dysfunction caused by oxidative stress impairs coronary collateral growth in ZOF.

Methods and results

Increased levels of reactive oxygen species were observed in aortic endothelium and smooth muscle cells in ZOF rats compared with ZLN rats. Reactive oxygen species levels were decreased by the mitochondria-targeted antioxidants MitoQuinone (MQ) and MitoTempol (MT) as assessed by MitoSox Red and dihydroethidine staining. Lipid peroxides (a marker of o

SUBMITTER: Pung YF 

PROVIDER: S-EPMC4013346 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature