A loop-mediated isothermal amplification assay for the visual detection of duck circovirus.

ABSTRACT:

Background

Duck circovirus (DuCV) infection in farmed ducks is associated with growth problems or retardation syndromes. Rapid identification of DuCV infected ducks is essential to control DuCV effectively. Therefore, this study aims to develop of an assay for DuCV to be highly specific, sensitive, and simple without any specialized equipment.

Methods

A set of six specific primers was designed to target the sequences of the Rep gene of DuCV, and A loop-mediated isothermal amplification (LAMP) assay were developed and the reaction conditions were optimized for rapid detection of DuCV.

Results

The LAMP assay reaction was conducted in a 62°C water bath condition for 50 min. Then the amplification products were visualized directly for color changes. This LAMP assay is hig

SUBMITTER: Xie L 

PROVIDER: S-EPMC4013541 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature