Transcriptional analysis of apoptotic cerebellar granule neurons following rescue by gastric inhibitory polypeptide.

ABSTRACT: Apoptosis triggered by exogenous or endogenous stimuli is a crucial phenomenon to determine the fate of neurons, both in physiological and in pathological conditions. Our previous study established that gastric inhibitory polypeptide (Gip) is a neurotrophic factor capable of preventing apoptosis of cerebellar granule neurons (CGNs), during its pre-commitment phase. In the present study, we conducted whole-genome expression profiling to obtain a comprehensive view of the transcriptional program underlying the rescue effect of Gip in CGNs. By using DNA microarray technology, we identified 65 genes, we named survival related genes, whose expression is significantly de-regulated following Gip treatment. The expression levels of six transcripts were confirmed by real-time quantitative polymeras

SUBMITTER: Maino B 

PROVIDER: S-EPMC4013584 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature