Identification, characterization, and expression of a unique secretory lipase from the human pathogen Leishmania donovani.

ABSTRACT: Lipases have been implicated to be of importance in the life cycle development, virulence, and transmission of a variety of parasitic organisms. Potential functions include the acquisition of host resources for energy metabolism and as simple building blocks for the synthesis of complex parasite lipids important for membrane remodeling and structural purposes. Using a molecular approach, we identified and characterized the structure of an LdLip3-lipase gene from the primitive trypanosomatid pathogen of humans, Leishmania donovani. The LdLip3 encodes a approximately 33 kDa protein, with a well-conserved substrate-binding and catalytic domains characteristic of members of the serine lipase-protein family. Further, we showed that LdLip3 mRNA is constitutively expressed by both the insect vect

SUBMITTER: Shakarian AM 

PROVIDER: S-EPMC4014072 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature